Fluorescent Probe for the Monitoring of Plasma Membrane Hydration

Subcellular microenvironment plays a crucial role in maintaining cell homeostasis being hydration an essential parameter to monitor the variations of lipid organization biological membranes. Fluorescent microenvironment-sensitive probes are stimuli-responsive molecules and able change their fluorescence profile according properties (polarity, hydration, microviscosity tension). Here we present organic fluorescent probe, NPM-1, as new microenvironmental dye with selective plasma membrane localization live cells. Using high-resolution confocal microscopy spectroscopy, explored polarity during formation apoptotic bodies real time. To have clear understanding variations, analogue probe localize cytosol nucleoli was also synthetized, CellPol-1. Then, were subcellular body dynamics.